Isothiocyanates (ITCs) contain anti-cancer agents that may provide the solution for natural anti-cancer treatment with fewer side effects for prostate cancer treatment. GMG-ITC was reported to have arrays of biological activities including anti-inflammatory, anti-oxidant, anti-microbial and anti-ulcer. GMG-ITC is a hydrolysis product of a rare glucosinolate known as glucomoringin (GMG) which was isolated from the seed of Moringa oleifera Lam. However, there has been a lack of study on glucomoringin isothiocyanate (GMG-ITC) ( Figure 1) against prostate cancer cell lines to determine its cytotoxic effect. The ITCs showed cytotoxic effects towards breast, liver, and lung cancer via modulation of MEK/ERK, PI3K/AKT, and Nrf2 intrinsic and extrinsic apoptosis pathways. Glucosinolates, which are derived from isothiocyanates (ITC) such as benzyl isothiocyanate (BITC), allyl isothiocyanate (AITC), and phenylethyl isothiocyanate (PEITC), have been reported for their protective effects against cancer cells. oleifera seeds could be useful as a future cytotoxic agent against prostate cancer. These phenomena suggest that GMG-ITC from M. It is concluded that apoptosis induction was observed in PC-3 cells treated with GMG-ITC. Early apoptosis proteins (JNK, Bad, Bcl2, and p53) were significantly upregulated upon GMG-ITC treatment. GMG-ITC also activates apoptotic genes including caspase, tumor suppressor gene (p53), Akt/MAPK, and Bax of the proapoptotic Bcl family. Moreover, GMG-ITC induced a time-dependent G2/M phase arrest, with reduction of 39.1% in the PC-3 cell line. Additionally, Annexin V assay showed proportion of cells in early and late apoptosis upon exposure to GMG-ITC in a time-dependent manner. Morphological changes as a result of GMG-ITC-induced apoptosis showed chromatin condensation, nuclear fragmentation, and membrane blebbing. GMG-ITC inhibited cell proliferation in the PC-3 cell line with IC50 value 3.5 µg/mL. Therefore, GMG-ITC was evaluated for its cytotoxicity against the PC-3 prostate cancer cell line and its potential to induce apoptosis. The plant contains glucomoringin isothiocyanate (GMG-ITC) with therapeutic potential against various cancer cells. Moringa oleifera Lam is often used in traditional medicine for the treatment of various illnesses. Inhibition of several protein pathways involved in cancer cell regulation is a necessary key in the discovery of cancer chemotherapy.
0 kommentar(er)
